Chloroquine transfection protocol

Discussion in 'Northwest Pharmaceuticals Canada' started by PaintBoy, 14-Mar-2020.

  1. Torrero2525 New Member

    Chloroquine transfection protocol


    This procedure can be modified for alternative packaging cell lines or transfection reagents. Once produced, lentivirus can be used for a variety of downstream applications such as stable-cell line generation.

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    This protocol is a modified version of a published method 4, in which calcium phosphate–based transfection methods for Chinese hamster ovary cells and the 293 line of human embryonic kidney. Protocol for Lentivial Vector LV Production 2nd Generation Packaging Lenti virus can be packaged by any transfection reagent. In this protocol, we describe two methods a calcium phosphate precipitation method and an iMFectin Poly DNA method. Chloroquine diphosphate 52 mg Deionized, distilled H 2 O 1 mL Dissolve 52 mg of chloroquine diphosphate in 1 mL of deionized, distilled H 2 O. Sterilize the solution by passing it through a 0.22-µm filter. Store the filtrate in foil-wrapped tubes at −20°C.

    Test a variety of brands and lots of FBS to find one suitable with your protocols. Last Upload: August 26, 2016 *Pro-Tip* Different brands and lots of FBS can promote or inhibit transfection.

    Chloroquine transfection protocol

    Calcium phosphate–mediated transfection of eukaryotic., Protocol for Lentivial Vector LV Production 2nd Generation.

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  6. Transfection Experiment Transfection Efficacy Reagent Volume Tissue Culture Grade Chloroquine Diphosphate These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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    The following are the protocols I've gathered *4-6 hours after transfection reaction has been applied to cells, gently remove media and replace with 10% glycerol or DMSO for 2-3 minutes. Remove and feed with complete media various references. *1 hour before transfection, replace cDMEM with cDMEM + 25 mM chloroquine. If chloroquine is not used, it is unnecessary to change the medium prior to transfection. On some occasions, we have obtained slightly improved transfection efficiencies by adding the chloroquine to a 11 mixture of 293 conditioned media obtained from any of the 293-based cell lines and fresh GM. Chloroquine is used for treating amebiasis, rheumatoid arthritis, discoid and systemic lupus erythematosus. Application DNA intercalator. Also used to increase transfection efficiency. Chloroquine diphosphate salt has been used • in in vitro antiplasmodial assays • in transfection and infection assays • in autophagy inhibition

     
  7. bynat New Member

    Hydroxychloroquine is used to prevent or treat malaria infections caused by mosquito bites. Hydroxychloroquine Plaquenil Side Effects & Dosage for Malaria How Effective is Hydroxychloroquine, Know It’s Dosage, Side. Hydroxychloroquine - Wikipedia
     
  8. Sensey Well-Known Member

    Plaquenil Hydroxychloroquine for Lupus - LupusCorner Long-term use of Plaquenil is associated with damage to the retina in the eye, the portion of the eyeball that is sensitive to light. It is important to see an ophthalmologist before starting Plaquenil to establish a baseline. And, you should have regular eye screenings the entire time you take the medication.

    Treating Lupus with Anti-Malarial Drugs Johns Hopkins.
     
  9. Floribunda XenForo Moderator

    Medicines for the Prevention of Malaria While. - cdc.gov Effects of chloroquine? Chloroquine is a relatively well-tolerated. medicine. The most common adverse reactions reported are stomach pain, nausea, vomiting, and headache. These side effects can often be lessened by taking chloroquine with food. Chloroquine may also cause itching in some people. All medicines may have some side effects.

    CDC - Malaria - Chloroquine Shortage